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2026年山东高考--基因工程的习题 这两天研究山东的高考题，今年的基因工程这道题真整体题干简短，图解简单，第（1）（4）题比较容易，但是第（2）（3）题乍一看还真有点懵。 研究了好久发现，解题的关键第一步是：信息的获取，读明白该题构建重组载体的方法：②TdT分别对基因W和切开的载体进行同聚物加尾（从3´端加）；若载体酶切后产生的黏性末端为5´端突出，则需先用DNA聚合酶补平后再进行同聚物加尾。第二步是：根据限制酶的切割位点以及同聚物加尾的信息来构建模型，即每一种限制酶切割+同聚物加尾后的情况。进而确定限制酶。第三步：根据DNA聚合酶和DNA连接酶的功能进行载体和目的基因的连接。 山东2026年的这道高考题与2025年的题，都考查了补平以及DNA聚合酶和DNA连接酶的作用。
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