应用Burrlioz 2.0软件绘制物种敏感度分布曲线(SSDs)

Burrlioz 2.0是由澳大利亚联邦科学和工业研究组织(CSIRO)与澳大利亚政府环境部合作开发的用于研究的应用程序。Burrlioz 2.0是一个独立开源的应用程序，以R统计软件中的统计处理为基础，使研究者能够根据澳大利亚和新西兰的水质指南或其他水质标准评估水生生物对有毒物质的敏感度(触发极限值)。敏感度分布曲线(SSDs)是通过拟合生物毒性数据的累计概率分布,来表征生物对污染物的敏感性。本文重点讲解Burrlioz应用程序的获取与基础使用。

(1) 准备数据

准备某水域水生生物对Fe离子(化合物)的无观测效应浓度数据fe.csv。A列代表无观测效应浓度(NOEC)数据，B列代表毒物浓度数据对应的物种，C列代表B列物种数据的分类信息。即A2(2.61 mg/L)代表B2(species1物种)对Fe离子的无观测效应浓度，B2(species1物种)属于C2(fish分类)。A列必须被定义为数字类型！美国环境保护署的水生毒性数据库(https://cfpub.epa.gov/ecotox/)与相关研究文献数据是常见的水生生物毒性数据来源。

百度网盘链接：https://pan.baidu.com/s/1k3sReCx_AdSXG-RppCj8-A

提取码：1fd8

(2) 阅读Burrlioz使用要约

根据CSIRO官网的Burrlioz使用要约，要求用户同意并接受使用要约的全部内容才可以免费使用Burrlioz应用程序。访问CSIRO官网 https://research.csiro.au/software/burrlioz/，务必滑动网页仔细阅读Burrlioz使用要约；

(3) 下载Burrlioz

阅读完成，滑到网页底部，点击“Burrlioz 2.0”按钮，同意要约并下载Burrlioz 2.0软件程序包；

如果网页访问失败或Burrlioz包下载失败，为了不影响研究者的工作进度，也可以在下文阅读Burrlioz使用要约原文，并在公众号后台回复“Burrlioz”获取Burrlioz程序包及其使用文档。

使用要约原文

A LICENCE CONCERNING SOFTWARE KNOWN AS "Burrlioz" ("Licence")

IMPORTANT NOTES

Do not use Burrlioz until you have read and agreed to the terms of this Licence.

The Commonwealth Scientific and Industrial Research Organisation, Australia ("CSIRO") in cooperation with the Australian Government Department of Environment has developed Burrlioz 2.0 for research purposes.

DEFINITION

The Burrlioz package (referred to herein as "Burrlioz") comprises executable files, associated software libraries and readme documentation.

OFFER

CSIRO and the Commonwealth offers you a right to use Burrlioz in accordance with the terms of this Licence ("Offer").

ACCEPTANCE

If you are employed and intend using Burrlioz in connection with your employment duties then you should only accept this Offer if your employer has authorised you to do so.

If you use the whole or any part of Burrlioz, then you (or, if you are employed, your employer) ("User") are deemed to have accepted the Offer.

TERMS

Nothing in this Licence grants any ownership of any rights to Burrlioz to any User.

Use of Burrlioz under this Licence is free of charge to the User.

The User may make copies of Burrlioz only as reasonably required for backup purposes. The User may not distribute Burrlioz. If the User becomes aware that any other person wishes to use Burrlioz, then the User should refer that person to the CSIRO download site.

The User must not use all or any part of Burrlioz in conjunction with any product or service (eg training or consulting) without a visible reference to the Burrlioz home page.

DISCLAIMER

Use of Burrlioz or any part of it is at the User's sole risk.

CSIRO and the Commonwealth does not warrant or make any representations:

(a)     that operation of Burrlioz will be-uninterrupted or that Burrlioz is error-free

(b)     regarding the results of any use of the whole or any part of Burrlioz;

(c)     as to the accuracy, reliability or content of any information service or goods obtained through any use of the whole or any part of Burrlioz.

CSIRO and the Commonwealth disclaims any and all liability for any injury, damage or loss (including, but not limited to, economic loss) howsoever arising out of any use of the whole or any part of Burrlioz.

TERMINATION

CSIRO and the Commonwealth may terminate this Licence immediately by written notice if the User breaches this Licence.

APPLICABLE LAW

This Licence will be interpreted according to the laws of the State of New South Wales, Australia.

(4) 安装Burrlioz

Burrlioz下载完毕，解压缩Burrlioz.zip。双击setup.exe程序执行安装；

(5) 下载R3.0.1

因为Burrlioz程序是以R统计软件中的统计处理为基础的，所以需要安装R软件。访问网页https://cran-archive.r-project.org/bin/windows/base/old/3.0.1 获取R3.0.1软件安装包，或在公众号后台回复“Burrlioz”获取R3.0.1软件安装包；

(6) 安装R3.0.1

(7) 选择数据

打开Burrlioz软件，点击“Brovse”按钮，弹出新对话框，浏览文件夹，选择fe.csv文件，点击“打开”；

(8) 加载数据

点击“Load data”，弹出提示框，Toxicant填写研究毒物(Fe)，Unites选择合适的单位(milligrans per litre，mg/L)，点击“OK”加载数据；

(9) R运算

提示“R call completed”，点击 “确定”；

(10) 输出数据

提示“Read Success – see data windows to check”，点击 “确定”；

(11) 检查数据

通过数据窗口查看数据是否正确，也可以根据需求，修改右侧数据矩阵的Symbol code列的标记；

(12) 选择模型

点击“Fit options”按钮进入模型选项界面，包含与数据拟合和显示相关的选项。选项框显示当前数据适合什么模型，由内置程序识别确定，不能更改。选项框包括Log scale(对数变换)、95% CI bounds(绘制95%置信区间)、Nominated protection level on the graph(标注95%保护水平限值HC5)，即物种敏感度分布曲线(SSDs)的5%分位数，可以简单理解为限值是95%环境生物不受影响的毒物浓度水平。选项框允许用户设置最小x值，对解析低浓度值的更多细节很有用。选项框允许用户指定图例框的坐标,通常选择默认值。复选框与选项框允许用户选择使用不同的绘图符号与标签(在Input页面上定义)。用户需要选择适当的复选框(包含标签的列名)，选择直接在图上绘制标签或在Input页面上创建的符号表。选项框允许用户修改轴标签，使用的是R包plotmath的一种算法，此框中也给出了算法的简要描述。设置好所有选项后，点击“Run”按钮运行，提示“R call completed”，点击 “确定”；

(13) 绘制SSDs

点击“Graph”按钮进入绘图界面，查看物种敏感度分布曲线(SSDs)绘制效果。可见95%保护水平限值HC5=0.047 mg/L，即理论上，研究水域水体铁离子浓度小于0.047 mg/L可满足95%生物保护效果。根据需求继续修改模型参数，重新计算绘图。点击“Export as JPEG”按钮导出结果图，点击“Burrlioz report”按钮导出结果报告；

(14) 查看SSDs

物种敏感度分布曲线(SSDs)见下图；

(15) 查看报告

输出报告，结果报告见下图。报告提供了研究对象、报告时间、单位、模型、80%/90%/95%/99%保护水平的指导值(含95%置信区间)和物种敏感度分布曲线(SSDs)。